Works, Carmen
http://hdl.handle.net/10211.1/1507
2024-03-29T12:44:55ZIsolation of a Novel Chromium (III) Binding Protein from Bovine Liver Tissue After Chromium (VI) Exposure
http://hdl.handle.net/10211.1/1513
Isolation of a Novel Chromium (III) Binding Protein from Bovine Liver Tissue After Chromium (VI) Exposure
Peterson, Ryan L.; Banker, Kelly J.; Garcia, Thelma Y.; Works, Carmen
In the ongoing investigation into the biological importance and toxicity issues surrounding the bioinorganic chemistry of chromium,
the accepted literature procedure for the isolation of the biological form of chromium, low molecular weight chromium binding protein
(LMWCr) or chromodulin, was investigated for its specificity. When chromium(VI) is added to bovine liver homogenate, results presented
here indicate at least four chromium(III) binding peptides and proteins are produced and that the process is non-specific for
the isolation of LMWCr. A novel trivalent chromium containing protein (1) has been isolated to purity and initial characterization is
reported here. Chromium(III) identification was determined by optical spectroscopy and diphenylcarbazide testing. This chromium binding
protein has a molecular weight of 15.6 kDa, which was determined from both gel-electrophoresis and mass spectrometry. The protein
is comprised primarily of Asx, Glx, His, Gly/Thr, Ala, and Lys in a 1.00:2.51:0.37:2.09:0.39:1.17 ratio and is anionic at pH 7.4. In addition,
the protein binds approximately 2.5 chromium(III) ions per molecule.
Published by and copyright by Elsevier.
2008-01-01T00:00:00ZPhotochemical Nitric Oxide Precursors: Synthesis, Photochemistry, and Ligand Substitution Kinetics of Ruthenium Salen Nitrosyl and Ruthenium Salophen Nitrosyl Complexes
http://hdl.handle.net/10211.1/1508
Photochemical Nitric Oxide Precursors: Synthesis, Photochemistry, and Ligand Substitution Kinetics of Ruthenium Salen Nitrosyl and Ruthenium Salophen Nitrosyl Complexes
Works, Carmen; Jocher, Christopher J.; Bart, Gwen D.; Bu, Xianhui; Ford, Peter C.
Over the past decade there has been considerable interest
in the chemistry and biochemistry of nitric oxide (NO,
nitrogen monoxide), owing to the discovery of diverse roles
in mammalian biology, examples being as a bioregulatory
molecule in the blood pressure control and as a toxic agent
produced in immune response to pathogens. Numerous disease states have been coupled to the under- or overproduction
of NO, and as a consequence, there has been
considerable interest in biomedical strategies for the selective
delivery and for selective trapping of NO. For example,
such NO delivery has been claimed to have potential
therapeutic value in treatments of such cardiovascular events
as stroke and heart attack5a as well as in treatment of cancer.
In this context, ongoing studies here and elsewhere have been
concerned with the preparation and mechanistic evaluation
of compounds having the potential for photochemically
activated NO delivery to specific physiological targets. For
example, our experiments have shown that NO release induced by photolysis of iron thiol nitrosyl clusters absorbed
into V79 Chinese hamster cells enhances รง-radiation killing
of such cells under hypoxic (oxygen deficient) conditions.
Published by and copyright by American Chemical Society. The definitive edition is available at www.acs.org.
2002-01-01T00:00:00Z